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IB Biology HL - 2024 - Questionbank

1.2 - Ultrastructure of Eukaryotes & Prokaryotes

Cell Ultrastructure, Prokaryotic & Eukaryotic cells, Functions of Organelles, Binary Fission, Micrographs, Microscopes

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Question 1

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Which of the following is true of centrifugation?


  • A.\hspace{1em} It separates substances by density

  • B.\hspace{1em} It separates substances by charge

  • C.\hspace{1em} It separates substances by chemical composition

  • D.\hspace{1em} It separates substances by colour

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Question 2

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What is the name of the organelle on the surface of the rough endoplasmic reticulum that is needed to synthesise polypeptide chains?


  • A.\hspace{1em} Nucleus

  • B.\hspace{1em} Vesicle

  • C.\hspace{1em} Ribosome

  • D.\hspace{1em} Mitochondrion

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Question 3

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What type of cell is shown in the image?


BI0392ii [Source: Adapted from Itayba, Hebrew Wikipedia. (2016, March 1). Category: Electron Microscope Images. Wikimedia Commons. Retrieved May 19, 2022, from https://commons.wikimedia.org/wiki/File: Electron_microscopy.jpg Copyright under CC BY 3.0]


  • A. \hspace{1em} A eukaryotic cell because it contains mitochondria

  • B. \hspace{1em} A prokaryotic cell because it has a plasma membrane

  • C. \hspace{1em} A eukaryotic cell because it does not have a nucleus

  • D. \hspace{1em} A prokaryotic cell because it contains ribosomes

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Question 4

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Which of the following would be considered an organelle?


  • A.\hspace{1em} cell wall

  • B.\hspace{1em} vesicles

  • C.\hspace{1em} cytoskeleton

  • D.\hspace{1em} cytoplasm

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Question 5

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How do prokaryotic cells divide?


  • A. \hspace{1em} Mitosis

  • B. \hspace{1em} Meiosis

  • C. \hspace{1em} Binary fission

  • D. \hspace{1em} Budding

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Question 6

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These photographs show the same section of a human retina. What is the reason for the difference between the two diagrams?


BI0391_1.2 [Source: Nestl, A. (2012, January 17). File AMD Super resolution Cremer.png - Wikimedia Commons. Wikimedia commons. Retrieved June 16, 2022, from https://commons.wikimedia.org/wiki/File: Opthalmology_AMD_Super_Resolution_Cremer.png Copyright under CC BY 3.0]


  • A. \hspace{1em} The photograph on the right was taken using an electron microscope.

  • B. \hspace{1em} The photograph on the right is at a higher resolution.

  • C. \hspace{1em} The photograph on the right is at a higher magnification.

  • D. \hspace{1em} The photograph on the right shows the ultrastructure of the cells.

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Question 7

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Which of the following correctly describes the images produced by electron microscopes?

  • I.High resolution
    II.High magnification
    III.Coloured


  • A.\hspace{1em} I only

  • B.\hspace{1em} I and II only

  • C.\hspace{1em} II and III only

  • D.\hspace{1em} I, II and III

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Question 8

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What do the two labelled organelles have in common?

BI0397i_1.2 [Source: Adapted from Itayba, Hebrew Wikipedia. (2016, March 1). Category: Electron Microscope Images. Wikimedia Commons. Retrieved May 19, 2022, from https://commons.wikimedia.org/wiki/Category Copyright under CC BY 3.0]


  • A. \hspace{1em} They are surrounded by a single membrane

  • B. \hspace{1em} They contain cristae

  • C. \hspace{1em} They are surrounded by a double membrane

  • D. \hspace{1em} They contain starch grains

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Question 9

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Which organelle has a double membrane?


  • A. \hspace{1em} Rough endoplasmic reticulum

  • B. \hspace{1em} Golgi apparatus

  • C. \hspace{1em} Nucleus

  • D. \hspace{1em} Vacuole

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Question 10

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Which row in the table correctly identifies structures which are present (\checkmark) or absent (×\times) in prokaryotic and eukaryotic cells?


StructureProkaryotic cellsEukaryotic cells
\hspace{1em}A.\hspace{1em}80S ribosomes\checkmark×\times
\hspace{1em}B.\hspace{1em}Plasma membrane×\times\checkmark
\hspace{1em}C.\hspace{1em}Nucleus\checkmark×\times
\hspace{1em}D.\hspace{1em}Mitochondria×\times\checkmark

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Question 11

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Which part of the cell is labelled with the letter P?

BI0395 image [Source: Adapted from Itayba, Hebrew Wikipedia. (2016, March 1). Category: Electron Microscope Images. Wikimedia Commons. Retrieved May 19, 2022, from https://commons.wikimedia.org/wiki/File: Electron_microscopy.jpg. Copyright under CC BY 3.0]


  • A. \hspace{1em} Vacuole

  • B. \hspace{1em} Lysosome

  • C. \hspace{1em} Nucleoid

  • D. \hspace{1em} Nucleus

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Question 12

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Which structure found in a eukaryote has a double membrane?


  • A.\hspace{1em} Golgi apparatus

  • B.\hspace{1em} Mitochondrion

  • C.\hspace{1em} Lysosome

  • D.\hspace{1em} Endoplasmic reticulum

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Question 13

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Which of the following is not a function of the Golgi apparatus?


  • A. \hspace{1em} Protein synthesis

  • B. \hspace{1em} Protein packaging into vesicles

  • C. \hspace{1em} Protein modification

  • D. \hspace{1em} Lipid packaging into vesicles

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Question 14

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Which row in the table correctly identifies structures which are present (✓) or absent (✕) in prokaryotic and eukaryotic cells?


StructureProkaryotic cellsEukaryotic cells
A.\hspace{1em}70S Ribosomes in Cytoplasm
B.\hspace{1em}Cell Wall
C.\hspace{1em}Mitochondria
D.\hspace{1em}Naked DNA

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Question 15

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Below is a micrograph of a bacterium, Marinomonas arctica, which is found in Arctic sea ice.

BI1258 [Source: Gulf of Alaska Seamounts expedition, 2019. Marinomonas arctica.jpg. Wikimedia. Retrieved, September 25th 2023, from https://commons.wikimedia.org/wiki/File: Marinomonas_arctica.jpg. Copyright under Public Domain]

Which row contains features of prokaryotic cells such as Marinomonas arctica?


RibosomesCell wallDNA associated with proteins
A.\hspace{1em}80SYesNo
B.\hspace{1em}80SYesYes
C.\hspace{1em}70SNoNo
D.\hspace{1em}70SYesNo

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Question 16

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Which statement(s) is/are not true of eukaryotic or prokaryotic cells?

  • I. \hspace{1em} Prokaryotic cells have a compartmentalised structure.

  • II.\hspace{.8em} Only eukaryotic cells have a Golgi apparatus.

  • III.\hspace{.6em} Only eukaryotic cells have membrane-bound organelles.


  • A. \hspace{1em} II only

  • B. \hspace{1em} I only

  • C. \hspace{1em} II and III only

  • D. \hspace{1em} I, II and III

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Question 17

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The micrograph below shows part of an animal cell. Which statement is true about the parts labelled X and Y

BI0912 [©Source: Adapted from T.Voekler (August 1986). File: Nucleus of a chloride cell.jpg. Wikimedia Commons. Retrieved July 22nd, 2023, from https://commons.wikimedia.org/wiki/File: Nucleus_of_a_chloride_cell.jpg. CC-BY-SA-3.0]


  • A.\hspace{1em} Both X and Y are primarily composed of water and solutes.

  • B.\hspace{1em} Only X has a specialised function, and Y does not.

  • C.\hspace{1em} Only Y is an organelle, and X is not.

  • D.\hspace{1em} Both X and Y are found in prokaryotes.

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Question 18

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Which structure is found in both eukaryotic and prokaryotic cells?


  • A.\hspace{1em} Lysosome

  • B.\hspace{1em} Ribosome

  • C.\hspace{1em} Golgi apparatus

  • D.\hspace{1em} Nucleoid

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Question 19

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Identify the row which is correct regarding the components of cells.


ComponentIs it an organelle?Function
A.\hspace{1em}Cell wallYesStructural support and protection
B.\hspace{1em}CytoplasmNoProduction of ATP
C.\hspace{1em}Golgi apparatusYesProcesses, modifies and packages proteins and lipids
D.\hspace{1em}LysosomeNoDigests macromolecules, pathogens, debris and old organelles

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Question 20

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Mesophyll cells are found in plants. Plant cells have unique characteristics that distinguish them from any other cell type.

BI0575

[Source: Adapted from Zhang, N., Zhang, L., Zhao, L., Ren, Y., Cui, D., Chen, J., ... & Chen, F. (2017). iTRAQ and virus-induced gene silencing revealed three proteins involved in the cold response in bread wheat. Scientific reports, 7(1), 1-16.Retrieved March 27, 2022. From https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5548720/. Copyright under CC by 4.0]

In the micrograph above, one of the cell parts is labelled X. What is the name of this structure?


  • A.\hspace{1em} Chloroplast

  • B.\hspace{1em} Central vacuole

  • C.\hspace{1em} Cell wall

  • D.\hspace{1em} Starch granule

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Question 21

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[Maximum mark: 6]

The micrograph shows a transverse section through a leaf.

BI0798A [Source from Berkshire Community College Bioscience Image Library,2018. Angiosperm Morphology Mesophyll Arrangement in Ligustrum (36198182664).jpg. Wikimedia Commons. Retrieved 29th January 2023 from https://commons.wikimedia.org/wiki/File:Angiosperm_Morphology_Mesophyll_Arrangement_in_Ligustrum_(36198182664).jpg.Copyright under Creative Commons CC0 1.0]

    1. Identify an adaptation of this leaf for its function. [1]

    2. Identify the cell organelle that is found in particularly high numbers in the palisade mesophyll cells. [1]


This micrograph shows a transverse section through a pancreatic acinar cell. Acinar cells are found in the exocrine tissue of the pancreas.

BI0798B [Source: Adapted from Howard, L., 2006. Pancreatic acinar cells.jpg. Wikimedia Commons. Retrieved 29th January 2023 from https://commons.wikimedia.org/w/index.php?search=pancreatic+cells&title=Special&go=Go&type=image&haslicense=unrestricted. Copyright under Public Domain]

    1. Explain how the structure of this cell is related to the function of the synthesis of proteins for release from the cell. [2]

    2. State why an electron microscope is needed to view the ultrastructure of the acinar cells. [2]

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Question 22

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The electron micrograph below shows a cell of the bacterium Vibrio cholerae. Which of the labelled structures maintains the cell’s shape and protects the cell from lysing during osmosis?


BI0494 [Source: Blokesch, M. & Schoolnik, G.K. (2007). Serogroup Conversion of Vibrio cholerae in Aquatic Reservoirs. PLoS Pathog 3(6): e81. Retrieved March 15, 2022, from https://doi.org/10.1371/journal.ppat.0030081.Copyright under CC BY 4.0]

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Question 23

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Which of the following is not an advantage of cell compartmentalisation?


  • A.\hspace{1em} Ability to control the distribution of metabolites and enzymes

  • B.\hspace{1em} Improved nutrient storage

  • C.\hspace{1em} Reduced energy costs

  • D.\hspace{1em} Improved metabolic efficiency

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Question 24

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Which organelle is the site of protein synthesis?

A.B.
BI0398_ABI0398_B
C.D.
BI0398_CBI0398_D

[Source A: Adapted from Itayba, Hebrew Wikipedia. (2016, March 1). Category: Electron Microscope Images. Wikimedia Commons. Retrieved May 19, 2022, from https://commons.wikimedia.org/wiki/File: Electron_microscopy.jpg Copyright under CC BY 3.0.
Source B: Adapted from: Openstax. (2016, May 18). File:0313 endoplasmic reticulum B en.png - Wikimedia Commons. Wikimedia commons. Retrieved June 19, 2022, from https://commons.wikimedia.org/wiki/File:0313_Endoplasmic_Reticulum_b_en.png Copyright under CC BY 3.0.
Source C: and3k, & caper437. (2009, June 25). File: chloroplast in leaf of anemone sp Tem 85000x.png - Wikimedia Commons. Wikimedia commons. Retrieved June 19, 2022, from https://commons.wikimedia.org/wiki/File.png Copyright under CC BY 3.0
Source D: Murphy, F. (2016, August 10). Free picture: Thin, section, ebola, virus. PIXNIO. Retrieved June 19, 2022, from https://pixnio.com/science/microscopy-images/ebola-hemorrhagic-fever-virus/thin-section-containing-the-ebola-virus Copyright free]

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Question 25

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Which statement applies to a lysosome?


  • A.\hspace{1em} It usually fuses with a vacuole in the cytoplasm.

  • B.\hspace{1em} It participates in anabolic reactions of cellular components.

  • C.\hspace{1em} It forms from vesicles budding from the nucleus.

  • D.\hspace{1em} It is an organelle which lacks a membrane and contains enzymes.

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Question 26

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Which of the following processes occur in prokaryotic cells but not eukaryotic cells?


  • A.\hspace{1em} Transcription takes place in the cytoplasm

  • B.\hspace{1em} mRNA splicing

  • C.\hspace{1em} Regulation of transcription

  • D.\hspace{1em} Translation of mRNA into a polypeptide chain

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Question 27

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[Maximum mark: 10]

The photomicrograph below shows a cyanobacterium, Synechococcus elongatus.


BI0400 [Source: Adapted from Gonzalez, R., & Kerfeld, C. (2022, June 13). Synechococcus elongates. Wikipedia. Retrieved June 19, 2022, from https://en.wikipedia.org/wiki/Synechococcus_elongatus Copyright under CC BY 3.0]


    1. State whether the cell shown in the photomicrograph is eukaryotic or prokaryotic. Give a reason for your answer. [2]

    2. State two features found in both eukaryotic and prokaryotic cell structures. [2]

    3. Identify the type of microscope which was used to produce the microphotograph above. Give a reason for your answer. [2]

    4. Describe the function of X. [2]

  1. Compare and contrast the structure of the Golgi apparatus and rough endoplasmic reticulum. [2]

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Question 28

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[Maximum mark: 4]

The micrograph below shows E. coli cells.

BI0799A [Source: Adapted from Highton, P., 1968. E.coli image.jpeg. Wikimedia Commons. Retrieved 29th January 2023 from https://commons.wikimedia.org/wiki/File.coli_image.jpg. Copyright under Creative CommonsCC0 1.0]

  1. Identify the structure labelled I in the micrograph. [1]

  2. If the magnification of the image is x36,000 and the length of cell II in the image is 54 mm, calculate the actual length of cell II in micrometres (µm). [2]

  3. State one feature, other than the structure labelled I, that can be used to identify the cells in this micrograph as prokaryotic cells. [1]

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Question 29

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The following electron micrograph shows part of an exocrine pancreas cell. Which of the labelled structures is responsible for the production of vesicles for exocytosis?


BI0532 [Source: Adapted from Howard, L. (n.d.). File: Pancreatic cells - TEM.jpg. Wikipedia. Retrieved January, 3, 2023, from https://commons.wikimedia.org/wiki/File: Pancreatic_cells_-_TEM.jpg. Copyright under Public Domain]

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Question 30

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[Maximum mark: 10]

The diagram shows the process of phagocytosis.

BI0917 [Source: Mango Slices (June 2016). File: Phagocytosis.svg. Wikimedia Commons. Retrieved July 24th, 2023, from https://commons.wikimedia.org/wiki/File: Phagocytosis.svg. CC-BY-SA-4.0]

  1. On the diagram, label a lysosome using the letter L. [1]

  2. State, with a reason, whether a lysosome is an organelle or not. [1]

  3. Predict, with a reason, how a cell might be affected if the enzymes present in lysosomes were not stored within a phospholipid bilayer. [2]

  4. Describe how ultracentrifugation is used in the study of organelles. [2]

  5. Distinguish between prokaryotes and eukaryotes, in terms of compartmentalisation. [1]

  6. Explain how compartmentalisation is beneficial during phagocytosis. [3]

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Question 31

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[Maximum mark: 8]

The micrograph below shows Cyanobacteria, which contain chlorophyll.

BI1197-SL [Source: Adapted from Thompson L. and Whatson N. (2007, January 1). Cyanobacteria. Wikipedia. Retrieved on September 6, 2023, from https://en.wikipedia.org/wiki/Cyanobacteria#. Copyright under CCO]

  1. Using your knowledge and the micrograph, state two features that would indicate that this is a bacterium. [2]

  2. Outline the structural differences between animal and plant cells. [3]

  3. Explain how light energy is used in photosynthesis to carry out anabolic reactions. [3]

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Question 32

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[Maximum mark: 16]

  1. Draw a labelled diagram which shows the structure of an animal cell. [4]

  2. Outline the advantages of a compartmentalised structure in eukaryotes. [4]

  3. Compare and contrast the ultrastructure of E. coli and a palisade mesophyll cell. [7]

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Question 33

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Which of the following structures are not found in either animal or fungal cells?


  • A.\hspace{1em} Mitochondria

  • B.\hspace{1em} Vacuoles

  • C.\hspace{1em} Plastids

  • D.\hspace{1em} Centrioles

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Question 34

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[Maximum mark: 16]

  1. Outline the features of cells that do not conform to the cell theory. [3]

  2. Use named species to explain how unicellular organisms carry out processes of life. [4]

  3. Compare and contrast the structure of prokaryotic and eukaryotic cells. [8]

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Question 35

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Palisade mesophyll cells produce and release large amounts of carbohydrates. Which characteristics would you expect to observe in such cells?


  • A.\hspace{1em} A high density of mitochondria and a large central vacuole.

  • B.\hspace{1em} A high density of chloroplasts and an extensive Golgi apparatus.

  • C.\hspace{1em} A high density of chloroplasts and a large central vacuole.

  • D.\hspace{1em} A high density of mitochondria and an extensive Golgi apparatus.

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Question 36

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[Maximum mark: 16]

  1. Describe centrifugation and how developments in this technique allowed scientists to improve their understanding of the components of cells. [4]

  2. Distinguish between transcription and translation in prokaryotic and eukaryotic organisms. [4]

  3. Explain the advantages of cell compartmentalisation in eukaryotes. [7]

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